Details
An antibody specific to the PAT/pat protein molecule is immobilized on the microwell plates and second antibody
specific for PAT/pat molecule is conjugated with horse radish peroxidase (HRP).
When the sample extracts are added to the microtiter wells, the PAT/pat protein from the sample binds to the
antibody immobilized in the well. This binding is subsequently detected by the addition of the enzyme-labeled antibody.
After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to
the amount of PAT/pat protein present in the sample. The reaction is terminated by addition of stop solution.
Absorbance is measured on a plate reader.
Kit Specifications
SKU
AID 027
Species
Multi-species
Analysis Scope
Qualitative
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