Details
An antibody specific to the Cry3Bb protein molecule is immobilized on the microwell plates and the second antibody
specific for Cry3Bb protein is conjugated with horse radish peroxidase (HRP). When the sample extracts are
added to the microtiter wells, the Cry3Bb from the sample binds to the antibody immobilized in the well. This binding
is subsequently detected by addition of enzyme-labeled antibody. After a washing step, the substrate is added. The
enzymatic reaction and development of color are proportional to the amount of Cry3Bb present in the sample.
The reaction is terminated by addition of stop solution. Absorbance is then measured on a plate reader.
Kit Specifications
SKU
AID 053
Species
Corn
Analysis Scope
Qualitative
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