Details
In the test, cotton sample extracts are added to test wells coated with antibodies raised against Cry1Ac and
Cry2A proteins. Any Cry1Ac or Cry2A protein present in the sample extract binds to the antibodies. This is
detected by the addition of alkaline phosphatase - labeled Cry2A antibody or horseradish peroxidase-labeled
Cry1Ac antibody. After a simple wash step, the results of the Cry2A assay are visualized via the addition of a
pNPP substrate. Once the yellow color develops and is read, the wash step is repeated, and TMB substrate is
added. The Cry1Ac results are visualized via the development of the resulting blue color which, subsequently,
becomes yellow on the addition of stop solution.
Kit Specifications
SKU
AID 017
Species
Cotton
Analysis Scope
Qualitative
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