RiboShield® RNase Inhibitor

For storage of samples, we recommend using RiboShield® RNase Inhibitor at a 100x solution (i.e., the concentration of RNase inhibitor in the storage solution should be 0.4 U/μL). This final concentration can be exceeded without adversely affecting downstream enzymatic reactions, but it is generally unnecessary unless RNase contamination is suspected.

All PCRBIO products for one-step and two-step RT-PCR as well as for cDNA synthesis already contain RNase inhibitor, so there is no need to add RiboShield® RNase Inhibitor when using these products.

If using with other kits, we recommend adding 1µL (40U) RiboShield® RNase Inhibitor to a 20µL reaction mix. In cases where the RNA source is known to be highly contaminated with RNases, titration may be required to verify complete protection of ribonucleic acid.

RiboShield® RNase Inhibitor is designed for use in RNA-sensitive applications such as RT-qPCR, cDNA synthesis, and RNA-seq, where the presence of even a small amount of RNase can harm RNA quality and experimental results. Additionally, it can be added to extracted RNA to preserve RNA quality in high-risk areas for RNase contamination or in RNA extraction solutions like those used in the study by Pereira et al., 2010, to enhance RNA integrity preservation as you proceed through your workflow.

(Pereira MJB, Behera V, Walter NG (2010) Non-denaturing purification of folded RNAs while avoiding common folding homogenerity. PLOS ONE 5(9): e12953. https://doi.org/10.1371/journal.pone.0012953)

RiboShield® RNase Inhibitor acts as a shield against the degradation of target RNA that might occur due to ribonuclease contamination during RNA preparation.

RiboShield® RNase Inhibitor is a non-covalently binding protein-based inhibitor that blocks the activity of common and troublesome RNases, including eukaryotic neutral RNases (e.g., RNase A, B, and C). It does not inhibit RNase T1, T2, U1, U2, CL3, RNase I, and H.