ptALS1-3: 4 Samples (20g each) with Allergens in a simple Food Matrix (rice flour) are provided. All parameters are contained in 2 of 4 samples providing 2/3 and 1/6 of the new Action Levels (VITAL 4.0, 2024), respectively. The “action level” in mg/kg is calculated from the allergen protein reference dose per 100 g food portion. The positive detection of the 2/3 action level ensures that the action level can be detected safely even when the measurement uncertainty of the applied method is about 50%. While the 1/6 action level can proof the method performance at lower levels close to the LOQ.
The evaluation of results is strictly qualitative with indication of the obtained agreements with the consensus values of the participants and the spiking of samples. All gravimetrically spiked quantitative levels are given in the reports which enables a semi-quantitative estimation of results.
For analysis ELISA (+ Lateral Flow), PCR and LC/MS can be applied.
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