| CAT Number | Product | Size | Price |
|---|---|---|---|
| PB25.11-01 | qPCRBIO SyGreen® 1-Step Detect Lo-ROX | 100 x 20 μL Reactions | Contact us |
| PB25.11-03 | qPCRBIO SyGreen® 1-Step Detect Lo-ROX | 300 x 20 μL Reactions | Contact us |
| PB25.11-12 | qPCRBIO SyGreen® 1-Step Detect Lo-ROX | 1200 x 20 μL Reactions | Contact us |
| PB25.12-01 | qPCRBIO SyGreen® 1-Step Detect Hi-ROX | 100 x 20 μL Reactions | Contact us |
| PB25.12-03 | qPCRBIO SyGreen® 1-Step Detect Hi-ROX | 300 x 20 μL Reactions | Contact us |
| PB25.12-12 | qPCRBIO SyGreen® 1-Step Detect Hi-ROX | 1200 x 20 μL Reactions | Contact us |
| PB25.31-01 | qPCRBIO SyGreen® 1-Step Go Lo-ROX | 100 x 20 μL Reactions | Contact us |
| PB25.31-03 | qPCRBIO SyGreen® 1-Step Go Lo-ROX | 300 x 20 μL Reactions | Contact us |
| PB25.31-12 | qPCRBIO SyGreen® 1-Step Go Lo-ROX | 1200 x 20 μL Reactions | Contact us |
| PB25.32-01 | qPCRBIO SyGreen® 1-Step Go Hi-ROX | 100 x 20 μL Reactions | Contact us |
| PB25.32-03 | qPCRBIO SyGreen® 1-Step Go Hi-ROX | 300 x 20 μL Reactions | Contact us |
| PB25.32-12 | qPCRBIO SyGreen® 1-Step Go Hi-ROX | 1200 x 20 μL Reactions | Contact us |
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Powered by Bioz © 2025Technical Specifications
Air-Dryable Probe 1-Step Mix
| Component | 600 reactions | 2000 reactions | 10,000 reactions | 100,000 reactions |
|---|---|---|---|---|
| 4x Air-Dryable Probe 1-Step Mix | 3 x 1 mL | 2 x 5 mL | 1 x 50 mL | 1 x 500 mL |
Air-Dryable Probe Mix
| Component | 600 reactions | 2000 reactions | 10,000 reactions | 100,000 reactions |
|---|---|---|---|---|
| 4x Air-Dryable Probe Mix | 3 x 1 mL | 2 x 5 mL | 1 x 50 mL |
Air-Dryable Probe 1-Step Mix
| Component | 600 reactions | 2000 reactions | 10,000 reactions | 100,000 reactions |
|---|---|---|---|---|
| 4x Air-Dryable Probe 1-Step Mix | 3 x 1 mL | 2 x 5 mL | 1 x 50 mL | 1 x 500 mL |
Air-Dryable Probe Mix
| Component | 600 reactions | 2000 reactions | 10,000 reactions | 100,000 reactions |
|---|---|---|---|---|
| 4x Air-Dryable Probe Mix | 3 x 1 mL | 2 x 5 mL | 1 x 50 mL |
Reaction Information
| Reaction Volume | Storage | |||
|---|---|---|---|---|
| 20 μL |
On receipt, the product should be stored between -30 and -20 °C. If stored correctly, the kit will retain full activity until the indicated expiry date. |
Additional Information
qPCRBIO SyGreen® 1-Step Kits are designed for rapid, highly specific, and ultra-sensitive real-time RT-PCR. We utilize the latest advances in reverse transcriptase technology and buffer chemistry to provide cDNA synthesis and real-time PCR in a single tube.
qPCRBIO SyGreen® 1-Step Kits can be used to quantify any RNA sample, including mRNA, total RNA, and viral RNA sequences. qPCRBIO SyGreen® 1-Step Detect is designed for sensitivity and is particularly suited for detecting targets with extremely low copy numbers. qPCRBIO SyGreen® 1-Step Go provides the earliest Ct and is optimized for fast and accurate results from high sample concentrations.
The kits include a modified thermostable and highly active MMLV reverse transcriptase and an advanced RNase inhibitor to prevent RNA degradation by contaminant RNase. The antibody-mediated hot-start technique enhances PCR amplification, prevents primer-dimer formation, and nonspecific products leading to improved sensitivity and specificity of the reaction. Combining the latest developments in polymerase technology with advanced buffer chemistry, we deliver market-leading performance with minimal or no optimization required.
Applications
Absolute quantification, Relative gene expression analysis, Extremely low copy target detection, qPCRBIO SyGreen 1-Step Detect recommended for sample amounts from 1pg - 10ng total RNA or >0.01pg mRNA per reaction, qPCRBIO SyGreen 1-Step Go recommended for sample amounts from 10pg - 100ng total RNA or >0.01pg mRNA per reaction.
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