Clara® Probe Purple Mix Clara® Probe Purple Mix Clara® Probe Purple Mix Clara® Probe Purple Mix Clara® Probe Purple Mix

Clara® Probe Purple Mix

Achieve the performance of Clara® Probe Mix with a non-reactive purple dye, easy on the eyes for visualizing samples. The detection of target DNA based on advanced qPCR probes has never been easier. Clara® Probe Purple Mix provides reliable, sensitive, and specific performance like our latest probe-based qPCR mix products. Moreover, this mix is supplemented with a special purple dye for easy visualization of samples during plate setup. This dye does not inhibit the PCR reaction and does not interfere with commonly used fluorescent probes, with minimal light reduction, ensuring a strong signal for accurate measurement. Features: High-concentration 4x mix, ideal for diverse, high-throughput assays Superior target DNA detection Reliable quantification for low-copy number samples Hot start antibody activation for increased sensitivity and specificity Improved speed and sensitivity for early target detection Non-reactive purple dye for easy visualization during setup
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CAT CodeProductSizePrice
PB20.65-01Clara® Probe Purple Mix Lo-ROX200 x 20 μL ReactionsContact us
PB20.65-03Clara® Probe Purple Mix Lo-ROX600 x 20 μL ReactionsContact us
PB20.65-05Clara® Probe Purple Mix Lo-ROX1000 x 20 μL Reactions (5 x 1 mL)Contact us
PB20.65-50Clara® Probe Purple Mix Lo-ROX10 000 x 20 μL Reactions (1 x 50 mL)Contact us
PB20.66-01Clara® Probe Purple Mix Hi-ROX200 x 20 μL ReactionsContact us
PB20.66-03Clara® Probe Purple Mix Hi-ROX600 x 20 μL ReactionsContact us
PB20.66-05Clara® Probe Purple Mix Hi-ROX1000 x 20 μL Reactions (5 x 1 mL)Contact us
PB20.66-50Clara® Probe Purple Mix Hi-ROX10 000 x 20 μL Reactions (1 x 50 mL)Contact us
PB20.67-01Clara® Probe Purple Mix No-ROX200 x 20 μL ReactionsContact us
PB20.67-03Clara® Probe Purple Mix No-ROX600 x 20 μL ReactionsContact us
PB20.67-05Clara® Probe Purple Mix No-ROX1000 x 20 μL Reactions (5 x 1 mL)Contact us
PB20.67-50Clara® Probe Purple Mix No-ROX10 000 x 20 μL Reactions (1 x 50 mL)Contact us
PB20.68-01Clara® Probe Purple Mix Separate-ROX200 x 20 μL ReactionsContact us
PB20.68-03Clara® Probe Purple Mix Separate-ROX600 x 20 μL ReactionsContact us
PB20.68-05Clara® Probe Purple Mix Separate-ROX1000 x 20 μL Reactions (5 x 1 mL)Contact us

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Technical Specifications

Clara® Probe Purple Mix Lo-ROX

Component 200 reactions 600 reactions 1000 reactions 10 000 reactions
4x Clara Probe Purple Mix Lo-ROX 1 x 1 mL 3 x 1 mL 5 x 1 mL 1 x 50 mL

Clara® Probe Purple Mix Hi-ROX

Component 200 reactions 600 reactions 1000 reactions 10 000 reactions
4x Clara Probe Purple Mix Hi-ROX 1 x 1 mL 3 x 1 mL 5 x 1 mL 1 x 50 mL

Clara® Probe Purple Mix No-ROX

Component 200 reactions 600 reactions 1000 reactions 10 000 reactions
4x Clara Probe Purple Mix No-ROX 1 x 1 mL 3 x 1 mL 5 x 1 mL 1 x 50 mL

Clara® Probe Purple Mix Separate-ROX

Component 200 reactions 600 reactions 1000 reactions
4x Clara Probe Purple Mix No-ROX 1 x 1 mL 3 x 1 mL 5 x 1 mL
50 μΜ ROX Additive 1 x 200 μL 1 x 200 μL 1 x 200 μL

Reaction Information

Reaction Volume Storage
20 μL On receipt, products should be stored between -30 and -20 °C.
If stored correctly, the kit will retain full activity until the specified expiration date.

Device Compatibility

This product is compatible with all standard and fast qPCR equipment. Use our qPCR Selection Tool to determine which ROX variant is compatible with your device.

Frequently Asked Questions (FAQs)

Yes, PCR products generated with these mixes have the same characteristics as PCR products generated with wild-type Taq polymerase. They can be sequenced or digested with restriction endonucleases following standard protocols. Products have 3′-d(A) overhang and can be used for TA cloning or can be blunt-ended or digested with restriction enzymes prior to cloning. For best results, we recommend purifying PCR products using any standard PCR cleanup kit.

No. Apart from ROX (if included in the kit), there are no other dyes in our mixes. Therefore, you can use any fluorophore-linked probe for your reaction.

Different products may result in different fluorescence levels. However, this does not affect the accuracy of quantification, and Ct values will not differ between products.

Yes, this storage buffer is compatible. EDTA will chelate some magnesium in the mix, but not significantly enough to affect the reaction.

The Clara® Probe Purple and Clara® Probe 1-Step Purple mixes contain passive reference dyes in multiple formulations, each with different reference dye concentrations:

Lo-ROX mixes (PB20.65 and PB25.85) contain 200 nM ROX.
Hi-ROX mixes (PB20.66 and PB25.86) contain 2 µM ROX.
No-ROX mixes (PB20.67 and PB25.87) do not contain ROX.

The Separate-ROX mixes (PB20.68 and PB25.88) include a separate 50 µM ROX tube. This allows you to choose the ROX concentration you wish to use.
You can use our qPCR selection tool in the resource dropdown menu to determine which of our mixes is best for your qPCR machine.

There are various options to consider when optimizing your reaction:

  • Reduce the annealing/extension temperature to 5 seconds
  • Increase the annealing/extension temperature from 60 to 65°C

Dilute sample DNA by starting with 5ng DNA and using a 10x dilution series. Besides running these on a gel to see if non-specific products persist, the performance of the reaction can be calculated by the qPCR equipment software after performing the sample dilutions. If the performance is between 90 – 110%, then the amplicon is doubling each cycle.

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