VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes VeriFi® Polymerase & Mixes

VeriFi® Polymerase & Mixes

VeriFi® Polymerase is a high-fidelity, versatile, and powerful proofreading DNA polymerase designed for all PCR applications where higher sequence accuracy is required. Improved DNA binding and enhanced performance result in shorter extension times, higher yields, and the ability to amplify longer and more difficult targets. Also available in a hot-start format. VeriFi® Polymerase was formerly known as PCRBIO VeriFi Polymerase. Features - High-temperature cycling – up to 100 °C for better denaturation of GC-rich samples - Increased PCR success rates with complex genomic templates (17.5 kb and above) - High yields under standard and fast PCR conditions (10-30 seconds/km) - Effective and specific amplification from challenging templates including GC and AT-rich sequences - 100 times higher fidelity than Taq DNA polymerase - Produces blunt-end PCR products - Available in a convenient 2x mix with an optional red dye for direct gel loading
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MS CATProductSizePrice
PB10.42-01VeriFi® Polymerase100 UnitsContact us
PB10.42-05VeriFi® Polymerase500 UnitsContact us
PB10.43-01VeriFi® Mix100 x 50 μL ReactionsContact us
PB10.43-05VeriFi® Mix500 x 50 μL ReactionsContact us
PB10.44-01VeriFi® Mix Red100 x 50 μL ReactionsContact us
PB10.44-05VeriFi® Mix Red500 x 50 μL ReactionsContact us

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Additional Information

What is VeriFi® Polymerase?

VeriFi® Polymerase is derived from Pfu DNA polymerase with 3’-5’ exonuclease (proofreading) activity. The high-fidelity enzyme is designed with proprietary mutations that significantly enhance its performance, resulting in shorter extension times (10-30 s/kb), higher yields, and the ability to amplify longer, more challenging targets, including eukaryotic genomes in excess of 17.5 kb. Previously, it was known as PCRBIO VeriFi Polymerase or PCRBIO VeriFi Mix (Red).

How does VeriFi® Polymerase ensure high accuracy?

The high fidelity and improved 3’-5’ exonuclease activity of VeriFi® Polymerase results in fidelity approximately 100 times higher than that of Taq DNA polymerase. This enzyme is ideal for applications requiring higher accuracy, such as cloning, directed mutagenesis, and sequencing. PCR products generated with this product line have blunt ends. VeriFi® Polymerase facilitates high-fidelity PCR with a wide range of targets and fragment sizes with little or no optimization needed. The enzyme is supplied with an advanced buffer system containing both dNTPs and Mg. For the most challenging templates, such as GC-rich sequences or those with complex secondary structures, users can add VeriMax Enhancer to the reaction mix to improve PCR performance. VeriFi® Polymerase is also available in a convenient ready-to-use 2x mix containing all reaction components except primers and template. Our 2x red mix includes a red dye suitable for direct loading and monitoring during agarose gel electrophoresis.

Applications

  • High fidelity PCR
  • Long-range PCR
  • Directed mutagenesis
  • Cloning
  • Sequencing
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