IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix IsoFast® Hot Start Bst Polymerase Colour & Mix

IsoFast® Hot Start Bst Polymerase Colour & Mix

IsoFast® Hot Start Bst Colour consists of colorimetric quantitative isothermal amplification enzyme components combining IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid green/purple screening. IsoFast® Hot Start Bst Polymerase is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus, Bst) DNA Polymerase. This colorimetric quantitative formulation is available as a pre-assembled mix, IsoFast® Hot Start Bst Colour Mix, for direct sample and primer input. Additionally, IsoFast® Hot Start Bst Polymerase Colour is available as a separate Bst DNA Polymerase system and color buffer, allowing full customization of the assay process and maximum flexibility in setup. This isothermal amplification system enables color readout based on DNA without the need for PCR, making it highly suitable for point-of-care diagnostics and field tests. Features: - Rapid color results for green/purple tests - AptaLock™ hot start for ultra-sensitive DNA target detection - Faster polymerization rate to shorten time to results (as little as 10 minutes) - Capable of detecting down to 3 target copies per μL - Suitable for both low temperature and room temperature setup - Speeds up and increases sensitivity for early target detection - High performance over a wide temperature range from 55-70 °C
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CAT No.ProductSizePrice
PB80.50-01IsoFast® Hot Start Bst Polymerase Colour1600 UnitsContact us
PB80.50-08IsoFast® Hot Start Bst Polymerase Colour8000 UnitsContact us
PB80.51-01IsoFast® Hot Start Bst Colour Mix100 x 25 μL ReactionsContact us
PB80.51-05IsoFast® Hot Start Bst Colour Mix500 x 25 μL ReactionsContact us

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Technical Specifications

IsoFast® Hot Start Bst Polymerase Colour

Component 1600 Units 8000 Units
IsoFast Hot Start Bst Polymerase 8 U/μL 1 x 200 μL 1 x 1 mL
10x IsoFast Colour Buffer A 1 x 500 μL 2 x 1.25 mL
5x IsoFast Buffer B 1 x 1 mL 3 x 1.7 mL

IsoFast® Hot Start Bst Colour Mix

Component 100 Reactions 500 Reactions
2x IsoFast Hot Start Bst Colour Mix 1 x 1.25 mL 5 x 1.25 mL

Reaction Information

Reaction Volume Storage
25 μL Upon receipt, products should be stored at temperatures between -30 and -20 °C. Properly stored, the product will retain full activity until the expiry date specified.

Frequently Asked Questions

Certain types of isothermal amplification strategies lend themselves to downstream applications, e.g. RCA (rolling circle amplification) or WGA (whole genome amplification) for NGS. IsoFast® Hot Start Bst reagents can be used for this type of amplification. However, the most common type of isothermal amplification, LAMP, generates multiple species of DNA products, making it unsuitable for cloning and impractical for NGS applications.

IsoFast® Hot Start Bst Polymerase, IsoFast® Hot Start Bst Mix, IsoFast® Hot Start Bst Colour Mix, and IsoFast® Hot Start Bst Polymerase Colour are all designed to amplify DNA and cDNA targets. They are not suitable for direct amplification of RNA targets.

For amplifying RNA targets, we recommend using IsoFast® Bst 1-Step Mix for direct 1-step isothermal amplification. Alternatively, we suggest a 2-step format where RNA is first converted into cDNA using one of our UltraScript® reagents, followed by cDNA amplification with the user's preferred IsoFast® Hot Start Bst reagent.

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