UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits UltraScript® Reverse Transcriptase & cDNA Synthesis Kits

UltraScript® Reverse Transcriptase & cDNA Synthesis Kits

UltraScript® Reverse Transcriptase is an enhanced, thermally stable reverse transcriptase enzyme, engineered from MMLV to accelerate and improve cDNA synthesis with accurate representation of transcripts. This enzyme enables highly sensitive reverse transcription of total RNA, poly A+ RNA, and viral RNA sequences, making it an ideal component in RT-qPCR based assays. Available as a standalone enzyme or as a ready mix of reverse transcriptase, buffer system, and optionally, an optimized blend of random hexamers with anchored oligo(dT) primers. All product formulations include a pre-mixed, potent RNase inhibitor to ensure the integrity of template RNA and minimize pipetting. This reverse transcriptase provides rapid, efficient, and sensitive cDNA synthesis with unbiased representation across a broad template RNA concentration range. Features - Unbiased representation of 5’ and 3’ ends of mRNA transcripts - Reduced RNase H activity - Thermally stable reverse transcription from 45 °C to 55 °C - Advanced RNase inhibitor - High cDNA yield from just 4 pg template RNA - Accurate reverse transcription of GC-rich templates - Sensitive detection of transcripts with low copy number - Advanced buffer chemistry including Mg and dNTPs - Available as a pre-mixed or separate oligo cDNA synthesis kit
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Catalog NumberProductSizePrice
PB30.11-02UltraScript® cDNA Synthesis Kit25 x 20 μL ReactionsContact us
PB30.11-10UltraScript® cDNA Synthesis Kit100 x 20 μL ReactionsContact us
PB30.12-01UltraScript® Reverse Transcriptase10 000 UnitsContact us
PB30.12-04UltraScript® Reverse Transcriptase40 000 UnitsContact us
PB30.13-01UltraScript® Reverse Transcriptase (8000 U/μL)80 000 UnitsContact us
PB30.15-02UltraScript® cDNA Synthesis Kit Separate Oligos25 x 20 μL ReactionsContact us
PB30.15-10UltraScript® cDNA Synthesis Kit Separate Oligos100 x 20 μL ReactionsContact us

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Additional Information

UltraScript® Reverse Transcriptase

UltraScript® Reverse Transcriptase is a modified reverse transcriptase (RTase) enzyme derived from MMLV, thermally stable, and highly active for cDNA synthesis. The reaction temperature can be increased up to 55 °C, providing higher specificity and efficient transcription of GC-rich RNA regions with high secondary structures. RTase is blended with an advanced RNase inhibitor to prevent RNA degradation by contaminant RNases. UltraScript® Reverse Transcriptase is not inhibited by ribosomal and transfer RNA, making synthesized RNA an ideal substrate. This enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers, and enhancers, optimized to produce high yield cDNA from as little as 4 pg of RNA. As oligos are not included, UltraScript® Reverse Transcriptase offers users convenience and flexibility in determining their own priming strategy depending on the analysis required. UltraScript® Reverse Transcriptase delivers exceptional performance with gene-specific primers, oligo(dT), and random hexamers to produce high-quality cDNA, ideal for numerous downstream applications. Our robust RTase is also available in a high concentration format and as a ready UltraScript® cDNA synthesis kit (formerly qPCRBIO cDNA synthesis kit), where the relative concentration of random hexamers and oligo(dT) have been optimized for cDNA production for use in real-time PCR experiments. This eliminates the need for user optimization of critical reverse transcription parameters, such as priming strategy, reverse transcriptase, buffer, and enhancers, and delivers unbiased, efficient, and sensitive cDNA synthesis every time. Read these Tips and Tricks to maximize your cDNA synthesis experiments. Learn about the best priming strategy to use depending on the starting RNA type you are using and the final application of your cDNA. For a quick summary of when each cDNA synthesis product is appropriate, use the reference table below:

Priming Type RNA Source Intended Use Suggested Product
Oligo(dT) & Random Hexamer Any qPCR, cloning, NGS PB30.11/PB30.15
Random Hexamer Prokaryotic, Archaeal qPCR, cloning, NGS PB30.15, (PB30.12 user needs to provide primer)
Oligo(dT) Eukaryotic qPCR, cloning, NGS PB30.15, (PB30.12 user needs to provide primer)
Gene-Specific Primer Any Targeted cloning, qPCR, NGS PB30.12 (user needs to provide primer)

Applications

  • Random hexamers, oligo(dT), and gene-specific primers
  • cDNA synthesis for real-time PCR analysis
  • cDNA synthesis for PCR analysis, cloning, library preparation, and Next Generation Sequencing
  • Low copy number transcripts
  • Viral RNA targets
  • miRNA targets
  • Efficient synthesis from total RNA or poly(A)+ RNA
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